LIC / Bio-organic Synthesis
Correlative light-electron microscopy (CLEM) is an imaging technique that allows for the localization of fluorescently labeled biomolecules within the ultrastructural context of the cell. In my research I will take advantage of the CLEM method to study cellular processes in immune cells. Among others I will use this approach to elucidate the composition of hydrolytic enzymes in the lysosomes of macrophages, and how this changes with lysosomal stress. Little is known about the specific composition of enzymes in lysosomes, and this research will help shed new light on this important part of the immune system.